محیط کشت کلروم آگار – CHROM agar

 

 

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Directions

chloram agar Suspend 16.0g per 500ml (32.0g/l) of distilled water and bring to the boil to dissolve completely. Reconstitute one vial SR0078E per 500ml medium or one vial SR0078H per 2 litres medium, as directed. Add the vial contents to Rose Bengal Chloramphenicol Agar Base (CM0549) and mix gently. Autoclave at 121°C for 5 minutes. Cool to 50°C, mix gently and pour into Petri dishes.

Description

Rose-Bengal Chloramphenicol Agar is a selective medium for the enumeration of yeasts and moulds from a wide variety of foodstuffs. The medium has a neutral pH and chloramphenicol is used as a selective agent to suppress the growth of bacteria. Several investigators have noted advantages in the use of media at neutral pH containing antibiotics1,2.

Rose-Bengal is taken up by mould and yeast colonies thereby assisting enumeration of small colonies3. Rose-Bengal also controls the size and height of mould colonies, such as Neurospora and Rhizopus spp. Over-growth of slow growing strains by more luxuriant species is thus prevented and plate counting is assisted.

chloram agar Suspend 16.0g per 500ml (32.0g/l) of distilled water and bring to the boil to dissolve completely. Reconstitute one vial SR0078E per 500ml medium or one vial SR0078H per 2 litres medium, as directed

Reconstitute one vial SR0078E per 500ml medium or one vial SR0078H per 2 litres medium, as directed. Add the vial contents to Rose Bengal Chloramphenicol Agar Base (CM0549) and mix gently. Autoclave at 121°C for 5 minutes

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